Pepsin Pepsin (EC 3.4.23.1) — Acidic Protein Hydrolysis Catalyst
High-activity Pepsin enzyme powder for reproducible acidic proteolysis in commercial workflows. Designed for cheese production optimization, brewing protein digestion, food-grade hydrolysis, and analytical processes requiring precise control. Activity 1:10,000 – 1:15,000 NF. Optimal pH 1.5–3.5 and 37°C–42°C. Sourced from porcine gastric mucosa. MOQ 1 kg. Global supply with consistent lot quality.
Certified ISO 9001
Product Specifications
01 Enzyme Name
Pepsin (EC 3.4.23.1)
02 Activity
1:10,000 – 1:15,000 NF (pepsin activity ratio)
03 pH Range
1.5 – 3.5
04 Temperature
37°C – 42°C
05 Appearance
White to pale yellow powder
06 Source
Porcine gastric mucosa
07 Shelf Life
24 months (sealed, refrigerated)
08 Packaging
1 kg / 5 kg / 25 kg
09 MOQ
1 kg
10 Certifications
USP/NF, EP, ISO 9001, HALAL
Applications
Pepsin catalyzes protein hydrolysis under strongly acidic conditions, delivering uniform performance for dairy processors, brewers, food manufacturers, analytical labs, and pharmaceutical production environments.
Cheese Rennet Alternative
Acid protease for milk-clotting in cheese manufacturing—used as partial rennet substitute where low pH proteolysis improves curd yield and texture.
Learn more →Digestive Supplements
Active ingredient in digestive enzyme supplements to support protein digestion—standardized NF/USP grades for reliable formulation.
Protein Sequencing (HDX-MS)
Pepsin digestion for hydrogen–deuterium exchange mass spectrometry sample preparation in structural biology and proteomics laboratories.
Antibody Fragmentation
Generates F(ab')2 fragments from IgG antibodies for diagnostic and therapeutic workflows in immunology.
Collagen & Gelatin
Facilitates extraction of acid-soluble collagen (pepsin-solubilized collagen) from animal tissues for gelatin processing and materials research.
Pharmaceutical Grade
USP/EP certified pepsin for pharmaceutical manufacturing—API production and quality control applications.
Learn About Pepsin
Industry guides covering process windows, supplier qualification, dosage, and applications — written for procurement and process engineers.
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Why Source From Us
Frequently Asked Questions
Q1 What is Pepsin (EC 3.4.23.1) used for?
Pepsin is applied in 🧀 cheese production as a rennet alternative, 💊 digestive supplement protein digestion, 🧬 HDX-MS sample prep, 🔬 antibody F(ab')2 generation, 🐄 collagen extraction, and 🧪 pharmaceutical manufacturing requiring acidic proteolysis.
Q2 What pH and temperature should I use for Pepsin?
Operate in pH 1.5–3.5 and 37°C–42°C for optimal activity and reproducible process outcomes.
Q3 How do I interpret the activity value 1:10,000 – 1:15,000 NF?
The NF activity ratio indicates proteolytic strength; select dosing based on pilot tests in your specific acidic protein hydrolysis process.
Q4 What is the recommended storage and shelf life?
Store sealed and refrigerated; shelf life is 24 months. Protect from moisture and adhere to COA/TDS handling guidelines.
Q5 What packaging sizes and minimum order quantities are available?
Available in 1 kg, 5 kg, and 25 kg packs. MOQ is 1 kg to accommodate pilot runs and full-scale supply.
Certifications & Standards
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