Pepsin Pepsin (EC 3.4.23.1) — Acidic Protein Hydrolysis Catalyst
Pepsin for manufacturers and labs that run protein work at low pH: cheese and dairy protein, food-grade hydrolysis, collagen extraction, antibody fragmentation and HDX-MS sample preparation. Activity 1:10,000 – 1:15,000 NF. Optimal pH 1.5–3.5 and 37°C–42°C. Sourced from porcine gastric mucosa. MOQ 1 kg. Request a quote with your product, quantity and destination.
Certified ISO 9001
Product Specifications
01 Enzyme Name
Pepsin (EC 3.4.23.1)
02 Activity
1:10,000 – 1:15,000 NF (pepsin activity ratio)
03 pH Range
1.5 – 3.5
04 Temperature
37°C – 42°C
05 Appearance
White to pale yellow powder
06 Source
Porcine gastric mucosa
07 Shelf Life
24 months (sealed, refrigerated)
08 Packaging
1 kg / 5 kg / 25 kg
09 MOQ
1 kg
10 Certifications
USP/NF, EP, ISO 9001, HALAL
Applications
Pepsin catalyzes protein hydrolysis under strongly acidic conditions. Dairy processors, food manufacturers, analytical labs, pharmaceutical producers and resellers buy it for the uses below; tell us your process and we quote the grade.
Cheese Rennet Alternative
Acid protease for milk coagulation in cheese manufacturing—used as a partial rennet substitute in specific cheese varieties; confirm curd yield and texture in your own vat trials.
Learn more →Supplement Formulations
Protease ingredient for capsule, tablet and powder enzyme blends—standardized NF/USP grades for consistent formulation.
Protein Sequencing (HDX-MS)
Pepsin cleavage for hydrogen–deuterium exchange mass spectrometry sample preparation in structural biology and proteomics laboratories.
Antibody Fragmentation
Generates F(ab')2 fragments from IgG antibodies for diagnostic and research reagent workflows in immunology.
Collagen & Gelatin
Facilitates extraction of acid-soluble collagen (pepsin-solubilized collagen) from animal tissues for gelatin processing and materials research.
Pharmaceutical Grade
USP/EP certified pepsin for pharmaceutical manufacturing—API production and quality control applications.
Learn About Pepsin
Industry guides covering process windows, supplier qualification, dosage, and applications — written for procurement and process engineers.
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Why Source From Us
Frequently Asked Questions
Q1 What is Pepsin (EC 3.4.23.1) used for?
Pepsin is applied in 🧀 cheese production as a rennet alternative, 💊 supplement and enzyme-blend formulation, 🧬 HDX-MS sample prep, 🔬 antibody F(ab')2 generation, 🐄 collagen extraction, and 🧪 pharmaceutical manufacturing requiring acidic proteolysis.
Q2 What pH and temperature should I use for Pepsin?
Operate in pH 1.5–3.5 and 37°C–42°C for optimal activity and reproducible process outcomes.
Q3 How do I interpret the activity value 1:10,000 – 1:15,000 NF?
The NF activity ratio indicates proteolytic strength; select dosing based on pilot tests in your specific acidic protein hydrolysis process.
Q4 What is the recommended storage and shelf life?
Store sealed and refrigerated; shelf life is 24 months. Protect from moisture and adhere to COA/TDS handling guidelines.
Q5 What packaging sizes and minimum order quantities are available?
Available in 1 kg, 5 kg, and 25 kg packs. MOQ is 1 kg to accommodate pilot runs and full-scale supply.
Q6 What should I send to get a quote?
The product and grade (for example NF/USP or EP), the quantity per order or per year, the destination country and your application. We reply within 24 hours with grade advice and a quote.
Q7 Do you quote distributors and resellers?
Yes. Distributors and resellers are quoted the same way: send the grade, quantity and destination. Packs are 1 kg, 5 kg and 25 kg, with a minimum order of 1 kg.
Certifications & Standards
Get a Free Sample & Quote
Share your process details and required grade. We will recommend suitable Pepsin activity, provide COA, and offer competitive pricing within 24 hours.
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