Direct from manufacturer

Pepsin Pepsin (EC 3.4.23.1) — Acid Protein-Hydrolyzing Enzyme

High-activity Pepsin for controlled protein breakdown under strongly acidic conditions. Ideal for protein digestion support, milk-clotting process development, and research workflows requiring reproducible acid proteolysis. Works best within recommended pH and temperature ranges. Activity 1:10,000 – 1:15,000 NF (pepsin activity ratio). Optimal pH 1.5–3.5 and 37°C–42°C. White to pale yellow powder. MOQ 1 kg. Ships worldwide.

15+ Years experience
80+ Countries shipped
Pepsin Enzyme Powder
Enzyme Name
Pepsin (EC 3.4.23.1)
Activity
1:10,000 – 1:15,000 NF (pepsin activity ratio)
pH Range
1.5 – 3.5
Temperature
37°C – 42°C
Appearance
White to pale yellow powder
Source
Porcine gastric mucosa
1:10,000 – 1:15,000 NF

ISO 9001 certified

Technical Data

Product Specifications

01 Enzyme Name

Pepsin (EC 3.4.23.1)

02 Activity

1:10,000 – 1:15,000 NF (pepsin activity ratio)

03 pH Range

1.5 – 3.5

04 Temperature

37°C – 42°C

05 Appearance

White to pale yellow powder

06 Source

Porcine gastric mucosa

07 Shelf Life

24 months (sealed, refrigerated)

08 Packaging

1 kg / 5 kg / 25 kg

09 MOQ

1 kg

10 Certifications

USP/NF, EP, ISO 9001, HALAL

Use Cases

Applications

Pepsin hydrolyzes proteins into peptides and smaller fragments under acidic conditions. Designed for consistent performance across supplement manufacturing, food processing, and advanced analytical and biomedical workflows.

1
🧀

Cheese Rennet Alternative

Milk-clotting enzyme for cheese production—used as a partial rennet substitute in specific cheese varieties where acid proteolysis supports curd development.

2
💊

Digestive Supplements

Active ingredient in digestive enzyme supplements to support protein digestion—standardized NF/USP grades for reliable formulation.

3
🧬

Protein Sequencing (HDX-MS)

Pepsin digestion for HDX-MS sample preparation in structural biology research, enabling controlled proteolysis for peptide mapping.

4
🔬

Antibody Fragmentation

Generates F(ab')2 fragments from IgG antibodies for diagnostic and therapeutic workflows in immunology.

5
🐄

Collagen & Gelatin

Supports extraction of acid-soluble collagen (pepsin-solubilized collagen) from animal tissues for medical and cosmetic use.

6
🧪

Pharmaceutical Grade

USP/EP certified pepsin for pharmaceutical manufacturing—API production and quality control applications.

Comparison

Why Source From Us

Feature
enzymepoint
Typical Supplier
NF/USP-aligned quality for acidic workflows
Reliable performance in low pH systems
Documented compliance & traceability
Flexible pack sizes & fast procurement
FAQ

Frequently Asked Questions

Q1 What is Pepsin (EC 3.4.23.1) used for?

Pepsin is used for 🧀 cheese rennet alternative applications, 💊 digestive supplement protein digestion support, 🧬 HDX-MS sample preparation, 🔬 antibody F(ab')2 generation, 🐄 pepsin-solubilized collagen workflows, and 🧪 pharmaceutical manufacturing where acidic proteolysis is required.

Q2 What pH and temperature should I use for Pepsin?

Use Pepsin in the pH 1.5–3.5 range and operate at 37°C–42°C for best activity and process consistency.

Q3 How do I interpret the activity value 1:10,000 – 1:15,000 NF?

The NF activity ratio reflects measured pepsin potency. Higher ratios generally indicate stronger proteolytic activity, but final dosing should be confirmed in your specific formulation or digestion protocol.

Q4 What is the recommended storage and shelf life?

Pepsin has a 24-month shelf life when sealed and refrigerated. Minimize moisture exposure and follow the storage guidance provided with your COA/TDS.

Q5 What packaging sizes and minimum order quantities are available?

Packaging is available in 1 kg, 5 kg, and 25 kg. MOQ starts at 1 kg, supporting both pilot studies and commercial scale supply.

Certifications & Standards

ISO 9001 HALAL Certified KOSHER Certified Food Grade Available Non-GMO Options Free Samples

Get a Free Sample & Quote

Tell us your application and required grade. We’ll recommend the right Pepsin activity level and send a free sample, COA, and competitive pricing within 24 hours.

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Response within 24 hours
Ships from China to 80+ countries
15+

Years experience

80+

Countries shipped

Free sample included with every qualified inquiry